Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
Product Information
15470-1-AP targets ALKBH7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7772 Product name: Recombinant human ALKBH7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-221 aa of BC004393 Sequence: MAGTGLLALRTLPGPSWVRGSGPSVLSRLQDAAVVRPGFLSTAEEETLSRELEPELRRRRYEYDHWDAAIHGFRETEKSRWSEASRAILQRVQAAAFGPGQTLLSSVHVLDLEARGYIKPHVDSIKFCGATIAGLSLLSPSVMRLVHTQEPGEWLELLLEPGSLYILRGSARYDFSHEILRDEESFFGERRIPRGRRISVICRSLPEGMGPGESGQPPPAC 相同性解析による交差性が予測される生物種 |
| Full Name | alkB, alkylation repair homolog 7 (E. coli) |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC004393 |
| Gene Symbol | ALKBH7 |
| Gene ID (NCBI) | 84266 |
| RRID | AB_2226855 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BT30 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ALKBH7(Alkylated DNA repair protein alkB homolog 7) is also named as ABH7, SPATA11 and belongs to the alkB family. This protein may be a dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Quantitative real-time PCR detectes variable ALKBH7 expression in all 16 tissues examined, with highest expression in pancreas, followed by spleen, prostate, ovary, and placenta(PMID:17979886). The gene encodes a 25 kDa protein with a 20 amino acid signal peptide.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ALKBH7 antibody 15470-1-AP | Download protocol |
| WB protocol for ALKBH7 antibody 15470-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.
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Cell Death Dis ALKBH7 drives a tissue and sex-specific necrotic cell death response following alkylation-induced damage.
| ||
