Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16837-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16837-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16837-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16837-1-AP (ALKBH5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16837-1-AP
IP result of anti-ALKBH5 (IP:16837-1-AP, 4ug; Detection:16837-1-AP 1:8000) with HeLa cells lysate 1280 ug.
IHC staining of human testis using 16837-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 16837-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse testis using 16837-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16837-1-AP (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16837-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16837-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16837-1-AP (ALKBH5 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ALKBH5 Polyclonal antibody

ALKBH5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16837-1-AP

Publications(196)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Synonyms

ALKBH 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, EC:1.14.11.53, RNA demethylase ALKBH5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (196)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human testis tissue, human lung cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 19 publications below
WB	See 174 publications below
IHC	See 27 publications below
IF	See 16 publications below
IP	See 1 publications below
CoIP	See 2 publications below
RIP	See 17 publications below

Product Information

16837-1-AP targets ALKBH5 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10516 Product name: Recombinant human ALKBH5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 57-383 aa of BC062339 Sequence: KRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAG 相同性解析による交差性が予測される生物種
Full Name	alkB, alkylation repair homolog 5 (E. coli)
Calculated molecular weight	458 aa, 51 kDa
Observed molecular weight	40-50 kDa
GenBank accession number	BC062339
Gene Symbol	ALKBH5
Gene ID (NCBI)	54890
RRID	AB_2242665
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6P6C2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

Protocols

Product Specific Protocols
IHC protocol for ALKBH5 antibody 16837-1-AP	Download protocol
IP protocol for ALKBH5 antibody 16837-1-AP	Download protocol
WB protocol for ALKBH5 antibody 16837-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Genet N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2. Authors - Yuan Li View Article
human	IF	Cell Res The m6A methylome of SARS-CoV-2 in host cells. Authors - Jun'e Liu KD Validated View Article
mouse	WB,RIP	Nat Cell Biol The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA. Authors - Xiang-Qian Gao View Article
human	WB	Nat Commun Circular RNA encoded MET variant promotes glioblastoma tumorigenesis Authors - Jian Zhong View Article
mouse,human	WB	Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma Authors - Weiwei Dai View Article
mouse	WB	Nat Commun ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice Authors - Tao Zhuang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16837-1-AP

WB analysis of HeLa using 16837-1-AP

WB analysis of HEK-293 using 16837-1-AP

IP experiment of HeLa using 16837-1-AP

IHC staining of human testis using 16837-1-AP

IHC staining of human testis using 16837-1-AP

IHC staining of mouse testis using 16837-1-AP

IHC staining of human lung cancer using 16837-1-AP

IHC staining of human lung cancer using 16837-1-AP

ALKBH5 Polyclonal antibody

ALKBH5 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

The m6A methylome of SARS-CoV-2 in host cells.

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma

ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice