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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALKBH3 Polyclonal antibody (12292-1-AP)

    WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    WB analysis of mouse kidney using 12292-1-AP (same clone as 12292-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    WB analysis of mouse heart using 12292-1-AP (same clone as 12292-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ALKBH3 Polyclonal antibody (12292-1-AP)

    WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human prostate cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human pancreas cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of rat stomach using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of mouse stomach using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human testis using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ALKBH3 Polyclonal antibody (12292-1-AP)

    IHC staining of human testis using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ALKBH3 Polyclonal antibody, PBS Only

ALKBH3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12292-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12292-1-PBS targets ALKBH3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2982

Product name: Recombinant human ALKBH3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 148-286 aa of BC015155

Sequence: MEPNPHWHPVLRTLKNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRVNLTFRTVYPDPRGAPW

相同性解析による交差性が予測される生物種
Full Name alkB, alkylation repair homolog 3 (E. coli)
Calculated molecular weight 286 aa, 33 kDa
Observed molecular weight 22-25 kDa, 33kDa
GenBank accession numberBC015155
Gene Symbol ALKBH3
Gene ID (NCBI) 221120
RRIDAB_11125161
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96Q83
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

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