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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ALG6 Polyclonal antibody (20753-1-AP)

    WB analysis of HeLa using 20753-1-AP (same clone as 20753-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20753-1-AP (ALG6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20753-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ALG6 Polyclonal antibody (20753-1-AP)

    WB analysis of HeLa using 20753-1-AP (same clone as 20753-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20753-1-AP (ALG6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20753-1-PBS in a different storage buffer formulation.

ALG6 Polyclonal antibody, PBS Only

ALG6 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 20753-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Asparagine linked glycosylation 6, Asparagine-linked glycosylation protein 6 homolog, Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase, Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase, Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20753-1-PBS targets ALG6 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14176

Product name: Recombinant human ALG6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-125 aa of BC001253

Sequence: GLTVRWTVSLNSYSGAGKPPMFGDYEAQRHWQEITFNLPVKQWYFNSSDNNLQYWGLDYPPLTAYHSLLCAYVAKFINPDWIALHTSRGYESQAHKLFMRTTVLIADLLIYI

相同性解析による交差性が予測される生物種
Full Name asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae)
Calculated molecular weight 507 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC001253
Gene Symbol ALG6
Gene ID (NCBI) 29929
RRIDAB_10693678
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y672
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ALG6 gene encodes an α-1,3-glucosyltransferase used to add the first glucose to the immature lipid-linked oligosaccharide (LLO) precursor. ALG6 has 14 transmembrane helices and two long loops (EL1 and EL4) that forms a large, hydrophilic cavity facing the ER lumen and a groove-shaped cavity facing the lipid bilayer (PMID: 21334936).

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