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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ALG3 Polyclonal antibody (28084-1-AP)

    WB analysis of HeLa using 28084-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28084-1-AP (ALG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using ALG3 Polyclonal antibody (28084-1-AP)

    IHC staining of mouse placenta using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using ALG3 Polyclonal antibody (28084-1-AP)

    IHC staining of mouse placenta using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using ALG3 Polyclonal antibody (28084-1-AP)

    IHC staining of mouse small intestine using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ALG3 Polyclonal antibody (28084-1-AP)

    IHC staining of human stomach cancer using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ALG3 Polyclonal antibody (28084-1-AP)

    IHC staining of mouse kidney using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALG3 Polyclonal antibody (28084-1-AP)

    IHC staining of mouse liver using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using ALG3 Polyclonal antibody (28084-1-AP)

    IHC staining of rat liver using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using ALG3 Polyclonal antibody (28084-1-AP)

    IHC staining of rat small intestine using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ALG3 Polyclonal antibody

ALG3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28084-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

Asparagine-linked glycosylation protein 3 homolog, CDGS4, D16Ertd36e, Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase, Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, human placenta tissue, mouse liver tissue
Positive IHC detected inmouse placenta tissue, rat small intestine tissue, rat liver tissue, mouse liver tissue, human stomach cancer tissue, mouse small intestine tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28084-1-AP targets ALG3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26382

Product name: Recombinant human ALG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-199 aa of BC002839

Sequence: MAAGLRKRGRSGSAAQAEGLCKQWLQRAWQERRLLLREPRYTLLVAACLCLAEVGITFWVIHRVAYTEIDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIRMAQNIFAVLYLATLLLVFLIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCC

相同性解析による交差性が予測される生物種
Full Name asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S. cerevisiae)
Calculated molecular weight 438 aa, 50 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC002839
Gene Symbol ALG3
Gene ID (NCBI) 10195
RRIDAB_3669644
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92685
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-1,3-mannosyltransferase (ALG3) encodes an endoplasmic reticulum-localized ALG3 family member that participates in N-glycan synthesis. ALG3 contributes to high mannose-type N-glycans in several cancers, and mannose-type N-glycan upregulation has been shown to be related to cancer progression. ALG3 is overexpressed in oral squamous cell carcinoma(PMID: 33084111), non-small cell lung cancer(PMID: 31899049), and breast cancer(PMID: 33931075) and hepatocellular carcinoma(PMID: 35782861).

Protocols

Product Specific Protocols
IHC protocol for ALG3 antibody 28084-1-APDownload protocol
WB protocol for ALG3 antibody 28084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Comput Biol Med

Deep pan-cancer analysis and multi-omics evidence reveal that ALG3 inhibits CD8+ T cell infiltration by suppressing chemokine secretion and is associated with 5-fluorouracil sensitivity

Authors - Zhixuan Wu
View Article
humanIHC

Cell Stress Chaperones

Inhibition of α-1,3-mannosyltransferase sensitizes head and neck squamous cell carcinomas to cetuximab via endoplasmic reticulum stress.

Authors - Yu Hao
View Article
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