Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
20290-1-AP targets ALG3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14059 Product name: Recombinant human ALG3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 241-299 aa of BC002839 Sequence: LQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLPEALFLHRAFHLALLTAHLT 相同性解析による交差性が予測される生物種 |
| Full Name | asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S. cerevisiae) |
| Calculated molecular weight | 438 aa, 50 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC002839 |
| Gene Symbol | ALG3 |
| Gene ID (NCBI) | 10195 |
| RRID | AB_2878663 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92685 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALG3 antibody 20290-1-AP | Download protocol |
| IHC protocol for ALG3 antibody 20290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Mol Biosci Expression of ALG3 in Hepatocellular Carcinoma and Its Clinical Implication. | ||
BMC Bioinformatics A novel glycosylation-related gene signature predicts survival in patients with lung adenocarcinoma | ||
J Exp Clin Cancer Res ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer. | ||
Cells ALG3 Promotes Peritoneal Metastasis of Ovarian Cancer through Increasing Interaction of α1,3-mannosylated uPAR and ADAM8
| ||
Heliyon Identification of ALG3 as a potential prognostic biomarker in lung adenocarcinoma
| ||
Metabolites Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response |