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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ALG1 Polyclonal antibody (12872-1-AP)

    WB analysis of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12872-1-AP (ALG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ALG1 Polyclonal antibody (12872-1-AP)

    IP experiment of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    IP result of anti-ALG1 (IP:12872-1-AP, 4ug; Detection:12872-1-AP 1:500) with HeLa cells lysate 3080 ug. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ALG1 Polyclonal antibody (12872-1-AP)

    IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ALG1 Polyclonal antibody (12872-1-AP)

    IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALG1 Polyclonal antibody (12872-1-AP)

    IF Staining of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12872-1-AP (ALG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ALG1 Polyclonal antibody, PBS Only

ALG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12872-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12872-1-PBS targets ALG1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3540

Product name: Recombinant human ALG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-464 aa of BC031095

Sequence: HNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPMREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVRTVLPLVMDT

相同性解析による交差性が予測される生物種
Full Name asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
Calculated molecular weight 464 aa, 53 kDa
Observed molecular weight 47-53 kDa
GenBank accession numberBC031095
Gene Symbol ALG1
Gene ID (NCBI) 56052
RRIDAB_2289541
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT22
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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