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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDOA Monoclonal antibody (67453-1-Ig)

    WB analysis using 67453-1-Ig (same clone as 67453-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67453-1-Ig (ALDOA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ALDOA Monoclonal antibody (67453-1-Ig)

    WB analysis using 67453-1-Ig (same clone as 67453-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67453-1-Ig (ALDOA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALDOA Monoclonal antibody (67453-1-Ig)

    IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ALDOA Monoclonal antibody (67453-1-Ig)

    IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALDOA Monoclonal antibody (67453-1-Ig)

    IF Staining of HeLa using 67453-1-Ig (same clone as 67453-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (67453-1-Ig, Clone: 1D6C2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

ALDOA Monoclonal antibody, PBS Only

ALDOA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67453-1-PBS
Clone No.1D6C2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67453-1-PBS targets ALDOA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17952

Product name: Recombinant human ALDOA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 2-364 aa of BC016800

Sequence: PYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY

相同性解析による交差性が予測される生物種
Full Name aldolase A, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC016800
Gene Symbol ALDOA
Gene ID (NCBI) 226
RRIDAB_2882687
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04075
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).