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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH7A1 Recombinant antibody (84871-4-RR)

    WB analysis using 84871-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84871-4-RR (ALDH7A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ALDH7A1 Recombinant monoclonal antibody (84871-4-RR)

    WB analysis of HepG2 using 84871-4-RR

    WB result of ALDH7A1 antibody (84871-4-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH7A1 transfected HepG2 cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using ALDH7A1 Recombinant antibody (84871-4-RR)

    IP experiment of HeLa using 84871-4-RR

    IP result of anti-ALDH7A1 (IP:84871-4-RR, 4ug; Detection:84871-4-RR 1:3000) with HeLa cells lysate 1200 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ALDH7A1 Recombinant antibody (84871-4-RR)

    IHC staining of human ovary cancer using 84871-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84871-4-RR (ALDH7A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ALDH7A1 Recombinant antibody (84871-4-RR)

    IHC staining of human ovary cancer using 84871-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84871-4-RR (ALDH7A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84871-4-RR

    Affinity and Kinetic Characterization of 84871-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84871-4-RR against Human ALDH7A1 were performed. The affinity constant is 0.253 nM.

  • Featured Product
  • KD/KO Validated

ALDH7A1 Recombinant monoclonal antibody

ALDH7A1 Uni-rAb® Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 84871-4-RR
Clone No.242363B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

242363B11, ALDH7A 1, Alpha-AASA dehydrogenase, Alpha-aminoadipic semialdehyde dehydrogenase, Antiquitin-1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, A549 cells, A431 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84871-4-RR targets ALDH7A1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0383

Product name: Recombinant human ALDH7A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 196-398 aa of BC002515

Sequence: SLISVAVTKIIAKVLEDNKLPGAICSLTCGGADIGTAMAKDERVNLLSFTGSTQVGKQVGLMVQERFGRSLLELGGNNAIIAFEDADLSLVVPSALFAAVGTAGQRCTTARRLFIHESIHDEVVNRLKKAYAQIRVGNPWDPNVLYGPLHTKQAVSMFLGAVEEAKKEGGTVVYGGKVMDRPGNYVEPTIVTGLGHDASIAHT

相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 7 family, member A1
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC002515
Gene Symbol ALDH7A1
Gene ID (NCBI) 501
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP49419
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDH7A1(Alpha-aminoadipic semialdehyde dehydrogenase) is also named as ATQ1 and belongs to the aldehyde dehydrogenase family. It plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.

Protocols

Product Specific Protocols
IHC protocol for ALDH7A1 antibody 84871-4-RRDownload protocol
IP protocol for ALDH7A1 antibody 84871-4-RRDownload protocol
WB protocol for ALDH7A1 antibody 84871-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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