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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH7A1 Polyclonal antibody (10368-1-AP)

    WB analysis using 10368-1-AP (same clone as 10368-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10368-1-AP (ALDH7A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10368-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ALDH7A1 Polyclonal antibody (10368-1-AP)

    WB analysis of HepG2 using 10368-1-AP (same clone as 10368-1-PBS)

    WB result of ALDH7A1 antibody (10368-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH7A1 transfected HepG2 cells. This data was developed using the same antibody clone with 10368-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ALDH7A1 Polyclonal antibody (10368-1-AP)

    WB analysis of mouse liver using 10368-1-AP (same clone as 10368-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10368-1-AP (ALDH7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10368-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ALDH7A1 Polyclonal antibody (10368-1-AP)

    WB analysis of mouse kidney using 10368-1-AP (same clone as 10368-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10368-1-AP (ALDH7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10368-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ALDH7A1 Polyclonal antibody (10368-1-AP)

    IHC staining of human ovary tumor using 10368-1-AP (same clone as 10368-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10368-1-AP (ALDH7A1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10368-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ALDH7A1 Polyclonal antibody (10368-1-AP)

    IHC staining of rat liver using 10368-1-AP (same clone as 10368-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10368-1-AP (ALDH7A1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10368-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALDH7A1 Polyclonal antibody (10368-1-AP)

    IF Staining of HeLa using 10368-1-AP (same clone as 10368-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDH7A1 antibody (10368-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10368-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ALDH7A1 Polyclonal antibody, PBS Only

ALDH7A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10368-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ALDH7A 1, Alpha-AASA dehydrogenase, Alpha-aminoadipic semialdehyde dehydrogenase, Antiquitin-1, ATQ1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10368-1-PBS targets ALDH7A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0383

Product name: Recombinant human ALDH7A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 196-398 aa of BC002515

Sequence: SLISVAVTKIIAKVLEDNKLPGAICSLTCGGADIGTAMAKDERVNLLSFTGSTQVGKQVGLMVQERFGRSLLELGGNNAIIAFEDADLSLVVPSALFAAVGTAGQRCTTARRLFIHESIHDEVVNRLKKAYAQIRVGNPWDPNVLYGPLHTKQAVSMFLGAVEEAKKEGGTVVYGGKVMDRPGNYVEPTIVTGLGHDASIAHT

相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 7 family, member A1
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC002515
Gene Symbol ALDH7A1
Gene ID (NCBI) 501
RRIDAB_2242476
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49419
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ALDH7A1(Alpha-aminoadipic semialdehyde dehydrogenase) is also named as ATQ1 and belongs to the aldehyde dehydrogenase family. It plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This protein has 4 isoforms produced by alternative splicing.

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