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  • Featured Product
  • KD/KO Validated

ALDH5A1 Monoclonal antibody

ALDH5A1 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Pig

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B9H10

Cat no : 67347-1-Ig

Synonyms

ALDH5A1, SSADH, SSDH

Validation Data Gallery

Applications:
  • WB analysis of L02 using 67347-1-Ig

    WB analysis of L02 using 67347-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67347-1-Ig

    WB analysis of HepG2 using 67347-1-Ig

    WB result of ALDH5A1 antibody (67347-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH5A1 transfected HepG2 cells.

  • WB analysis of HepG2 using 67347-1-Ig

    WB analysis of HepG2 using 67347-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig brain using 67347-1-Ig

    WB analysis of pig brain using 67347-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67347-1-Ig

    IHC staining of human liver cancer using 67347-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67347-1-Ig

    IHC staining of human liver cancer using 67347-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67347-1-Ig

    IHC staining of human liver cancer using 67347-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67347-1-Ig

    IHC staining of human liver cancer using 67347-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 67347-1-Ig

    IF Staining of human liver cancer using 67347-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ALDH5A1 antibody (67347-1-Ig, Clone: 1B9H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • FC experiment of A431 using 67347-1-Ig

    FC experiment of A431 using 67347-1-Ig

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ALDH5A1 (67347-1-Ig, Clone:1B9H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inL02 cells, HepG2 cells, pig brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Positive FC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67347-1-Ig targets ALDH5A1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse, Pig samples.

Tested Reactivity Human, Rat, Mouse, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALDH5A1 fusion protein Ag11155 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 5 family, member A1
Calculated molecular weight 535 aa, 57 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC034321
Gene symbol ALDH5A1
Gene ID (NCBI) 7915
RRIDAB_2882605
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ALDH5A1 antibody 67347-1-IgDownload protocol
IHC protocol for ALDH5A1 antibody 67347-1-IgDownload protocol
IF protocol for ALDH5A1 antibody 67347-1-IgDownload protocol
FC protocol for ALDH5A1 antibody 67347-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols