Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 17319-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17319-1-AP (ALDH5A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17319-1-AP
WB result of ALDH5A1 antibody (17319-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH5A1 transfected HepG2 cells.
IHC staining of human liver cancer using 17319-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 17319-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 17319-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 17319-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 17319-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17319-1-AP (ALDH5A1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 17319-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17319-1-AP (ALDH5A1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A431 using 17319-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ALDH5A1 Polyclonal antibody

ALDH5A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17319-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ALDH5A1, SSADH, SSDH

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

17319-1-AP targets ALDH5A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ALDH5A1 fusion protein Ag11293 相同性解析による交差性が予測される生物種
Full Name	aldehyde dehydrogenase 5 family, member A1
Calculated molecular weight	535 aa, 57 kDa
Observed molecular weight	51-59 kDa
GenBank accession number	BC034321
Gene Symbol	ALDH5A1
Gene ID (NCBI)	7915
RRID	AB_2224478
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P51649
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ALDH5A1 antibody 17319-1-AP	Download protocol
IHC protocol for ALDH5A1 antibody 17319-1-AP	Download protocol
IF protocol for ALDH5A1 antibody 17319-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice Authors - Si-Wei Rao View Article
human	IHC	Cancer Biol Ther Decreased expression of ALDH5A1 predicts prognosis in patients with ovarian cancer. Authors - Xun Tian View Article
mouse	WB	Neuropharmacology A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine Authors - Xiao-Hui Tang View Article
human,mouse	WB,IF	Aging (Albany NY) Mitochondrial-related genes PDK2, CHDH, and ALDH5A1 served as a diagnostic signature and correlated with immune cell infiltration in ulcerative colitis Authors - Qian Yang View Article
mouse	WB	Ecotoxicol Environ Saf 1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway Authors - Yuchen Qin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17319-1-AP

WB analysis of HepG2 using 17319-1-AP

IHC staining of human liver cancer using 17319-1-AP

IHC staining of human liver cancer using 17319-1-AP

IHC staining of human liver cancer using 17319-1-AP

IHC staining of human liver cancer using 17319-1-AP

IHC staining of human liver using 17319-1-AP

IHC staining of human liver using 17319-1-AP

IF Staining of A431 using 17319-1-AP

ALDH5A1 Polyclonal antibody

ALDH5A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice

Decreased expression of ALDH5A1 predicts prognosis in patients with ovarian cancer.

A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine

Mitochondrial-related genes PDK2, CHDH, and ALDH5A1 served as a diagnostic signature and correlated with immune cell infiltration in ulcerative colitis

1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway