Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, BxPC-3 cells, HepG2 cells |
| Positive IHC detected in | human adrenal gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
15746-1-AP targets ALDH3B2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8197 Product name: Recombinant human ALDH3B2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 92-385 aa of BC007685 Sequence: TGQLLEHKLDYIFFTGSPRVGKIVMTAATKHLTPVTLELGGKNPCYVDDNCDPQTVANRVAWFCYFNAGQTCVAPDYVLCSPEMQERLLPALQSTITRFYGDDPQSSPNLGRIINQKQFQRLRALLGCGRVAIGGQSNESDRYIAPTVLVDVQETEPVMQEEIFGPILPIVNVQSVDEAIKFINWQEKPLALYAFSNSSQVVNQMLERTSSGSFGGNEGFTYISLLSVPFGGVGHSGMGRYHGKFTFDTFSHHRTCLLAPSGLEKLKEIHYPPYTDWNQQLLRWGMGSQSCTLL 相同性解析による交差性が予測される生物種 |
| Full Name | aldehyde dehydrogenase 3 family, member B2 |
| Calculated molecular weight | 395 aa, 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC007685 |
| Gene Symbol | ALDH3B2 |
| Gene ID (NCBI) | 222 |
| RRID | AB_2242461 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48448 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ALDH3B2(aldehyde dehydrogenase family 3 member B2) is also named as ALDH8(aldehyde dehydrogenase 8) and belongs to the aldehyde dehydrogenase family. The ALDH3B2 gene contains an in-frame stop codon at the seventeenth codon position from the first methionine,suggesting that ALDH3B2 may be a pseudogene(PMID:15237855) and encodes the human salivary gland and placental ALDH(PMID: 22206977). This protein shares 83% sequence identity with ALDH3B1(PMID:18611112 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ALDH3B2 antibody 15746-1-AP | Download protocol |
| IHC protocol for ALDH3B2 antibody 15746-1-AP | Download protocol |
| WB protocol for ALDH3B2 antibody 15746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Aldehyde dehydrogenase 3B2 promotes the proliferation and invasion of cholangiocarcinoma by increasing Integrin Beta 1 expression
| ||
Int J Mol Sci Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma | ||
Biochem Pharmacol β-Elemene inhibits epithelial-mesenchymal transformation in non-small cell lung cancer by targeting ALDH3B2/RPSA axis
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