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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH1L1 Recombinant antibody (85980-2-RR)

    WB analysis using 85980-2-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 85980-2-RR (ALDH1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using ALDH1L1 Recombinant antibody (85980-2-RR)

    IF Staining of Neuro-2a using 85980-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ALDH1L1 antibody (85980-2-RR, Clone: 250565B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85980-2-RR

    Affinity and Kinetic Characterization of 85980-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85980-2-RR against Human ALDH1L1 were performed. The affinity constant is 2.04 nM.

ALDH1L1 Recombinant monoclonal antibody

ALDH1L1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85980-2-RR
Clone No.250565B12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

10 FTHFDH, 10-FTHFDH, ALDH1L 1, Cytosolic 10-formyltetrahydrofolate dehydrogenase, EC:1.5.1.6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IF/ICC detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85980-2-RR targets ALDH1L1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALDH1L1 fusion protein Ag11285 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 1 family, member L1
Calculated molecular weight 99 kDa
Observed molecular weight100 kDa
GenBank accession numberBC027241
Gene Symbol ALDH1L1
Gene ID (NCBI) 10840
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75891
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

Protocols

Product Specific Protocols
WB protocol for ALDH1L1 antibody 85980-2-RRDownload protocol
IF protocol for ALDH1L1 antibody 85980-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols