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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH1B1 Recombinant antibody (85426-4-RR)

    WB analysis using 85426-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85426-4-RR (ALDH1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85426-4-RR

    Affinity and Kinetic Characterization of 85426-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85426-4-RR against Human ALDH1B1 were performed. The affinity constant is below 1 pM.

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ALDH1B1 Recombinant antibody

ALDH1B1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85426-4-RR
Clone No.242944D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242944D1, Aldehyde dehydrogenase 5, Aldehyde dehydrogenase X, mitochondrial, ALDH5, ALDHX

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, HL-60 cells, mouse brain tissue, rat brain tissue, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85426-4-RR targets ALDH1B1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALDH1B1 fusion protein Ag7144 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 1 family, member B1
Calculated molecular weight 57 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC001619
Gene Symbol ALDH1B1
Gene ID (NCBI) 219
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

Protocols

Product Specific Protocols
WB protocol for ALDH1B1 antibody 85426-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols