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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ALDH1B1 Polyclonal antibody (15560-1-AP)

    WB analysis of HepG2 using 15560-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ALDH1B1 Polyclonal antibody (15560-1-AP)

    WB analysis of K-562 using 15560-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ALDH1B1 Polyclonal antibody (15560-1-AP)

    WB analysis of HepG2 using 15560-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ALDH1B1 Polyclonal antibody (15560-1-AP)

    WB analysis of mouse brain using 15560-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ALDH1B1 Polyclonal antibody (15560-1-AP)

    WB analysis of HepG2 using 15560-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ALDH1B1 Polyclonal antibody (15560-1-AP)

    WB analysis of K-562 using 15560-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ALDH1B1 Polyclonal antibody (15560-1-AP)

    IP experiment of mouse brain using 15560-1-AP

    IP result of anti-ALDH1B1 (IP:15560-1-AP, 4ug; Detection:15560-1-AP 1:20000) with mouse brain tissue lysate 2100 ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using ALDH1B1 Polyclonal antibody (15560-1-AP)

    IHC staining of human testis using 15560-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ALDH1B1 Polyclonal antibody (15560-1-AP)

    IHC staining of human testis using 15560-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ALDH1B1 Polyclonal antibody (15560-1-AP)

    IF Staining of HepG2 using 15560-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ALDH1B1 antibody (15560-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ALDH1B1 Polyclonal antibody

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15560-1-AP
Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:1.2.1.3, ALDHX, ALDH5, Aldehyde dehydrogenase X, mitochondrial, Aldehyde dehydrogenase 5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15560-1-AP targets ALDH1B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7144

Product name: Recombinant human ALDH1B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 168-517 aa of BC001619

Sequence: FCFTRHEPVGVCGQIIPWNFPLVMQGWKLAPALATGNTVVMKVAEQTPLSALYLASLIKEAGFPPGVVNIITGYGPTAGAAIAQHMDVDKVAFTGSTEVGHLIQKAAGDSNLKRVTLELGGKSPSIVLADADMEHAVEQCHEALFFNMGQCCCAGSRTFVEESIYNEFLERTVEKAKQRKVGNPFELDTQQGPQVDKEQFERVLGYIQLGQKEGAKLLCGGERFGERGFFIKPTVFGGVQDDMRIAKEEIFGPVQPLFKFKKIEEVVERANNTRYGLAAAVFTRDLDKAMYFTQALQAGTVWVNTYNIVTCHTPFGGFKESGNGRELGEDGLKAYTEVKTVTIKVPQKNS

相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 1 family, member B1
Calculated molecular weight 57 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC001619
Gene Symbol ALDH1B1
Gene ID (NCBI) 219
RRIDAB_2224162
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP30837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).

Protocols

Product Specific Protocols
IF protocol for ALDH1B1 antibody 15560-1-APDownload protocol
IHC protocol for ALDH1B1 antibody 15560-1-APDownload protocol
IP protocol for ALDH1B1 antibody 15560-1-APDownload protocol
WB protocol for ALDH1B1 antibody 15560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Nat Commun

Compression-induced NF-κB activation sustains tumor cell survival in confinement by detoxifying aldehydes and promotes metastasis.

Authors - Bing Liu
  • KD Validated
View Article
mouseWB

Mol Cell

Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.

Authors - Nina Oberbeck
View Article

Adv Sci (Weinh)

BIN1 and ALDH1B1 Deficiency in Colonic Smooth Muscle Drives Mitochondrial Dysfunction and Fibrosis in Slow-Transit Constipation.

Authors - Jianbo Liu
View Article
mouseIHC

J Transl Med

Identification and experimental validation of KMO as a critical immune-associated mitochondrial gene in unstable atherosclerotic plaque

Authors - Fu-Jun Liao
View Article
mouseIHC

Oncogene

Lfng-expressing centroacinar cell is a unique cell-of-origin for p53 deficient pancreatic cancer

Authors - Wen-Cheng Chung
View Article
humanIHC

Apoptosis

Metabolism-related ALDH1B1 acts as potential predictor and therapeutic target for primary gastrointestinal diffuse large B-cell lymphoma

Authors - Qiqi Qiao
View Article
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