Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
29373-1-AP targets ALDH1A3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31100 Product name: Recombinant human ALDH1A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 40-124 aa of BC069274 Sequence: WHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGK 相同性解析による交差性が予測される生物種 |
| Full Name | aldehyde dehydrogenase 1 family, member A3 |
| Calculated molecular weight | 512 aa, 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC069274 |
| Gene Symbol | ALDH1A3 |
| Gene ID (NCBI) | 220 |
| RRID | AB_2918287 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P47895 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
ALDH1A3 is the predominant ALDH isozyme that participates in various physiological processes in human cells. Growing evidence has shown that ALDH1A3 exhibits high activity in cancer stem cells (CSCs) and influences a diverse range of biological characteristics within these cells. (PMID: 30958800,PMID: 32612951)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ALDH1A3 antibody 29373-1-AP | Download protocol |
| WB protocol for ALDH1A3 antibody 29373-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
