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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALCAM Polyclonal antibody (32402-1-AP)

    WB analysis using 32402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32402-1-AP (ALCAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALCAM Polyclonal antibody (32402-1-AP)

    IHC staining of mouse liver using 32402-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALCAM Polyclonal antibody (32402-1-AP)

    IHC staining of mouse liver using 32402-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ALCAM Polyclonal antibody (32402-1-AP)

    IHC staining of mouse brain using 32402-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ALCAM Polyclonal antibody (32402-1-AP)

    IHC staining of mouse brain using 32402-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32402-1-AP (ALCAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ALCAM Polyclonal antibody

ALCAM Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32402-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen, Protein DM-GRASP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue, rat liver tissue
Positive IHC detected inmouse liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32402-1-AP targets ALCAM in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name activated leukocyte cell adhesion molecule
Calculated molecular weight65 kDa
Observed molecular weight90-110 kDa
GenBank accession numberNM_009655.3
Gene Symbol Alcam
Gene ID (NCBI) 11658
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ61490
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Protocols

Product Specific Protocols
WB protocol for ALCAM antibody 32402-1-APDownload protocol
IHC protocol for ALCAM antibody 32402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols