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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALAD Polyclonal antibody (31686-1-AP)

    WB analysis using 31686-1-AP (same clone as 31686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31686-1-AP (ALAD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using ALAD Polyclonal antibody (31686-1-AP)

    WB analysis of U-251 using 31686-1-AP (same clone as 31686-1-PBS)

    WB result of ALAD antibody (31686-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALAD transfected U-251 cells. This data was developed using the same antibody clone with 31686-1-PBS in a different storage buffer formulation.

ALAD Polyclonal antibody, PBS Only

ALAD Polyclonal Antibody for WB, Indirect ELISA
Cat No. 31686-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ALADH, Delta aminolevulinic acid dehydratase, Porphobilinogen synthase

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31686-1-PBS targets ALAD in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35649

Product name: Recombinant human ALAD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 151-330 aa of NM_000031.5

Sequence: AEVALAYAKAGCQVVAPSDMMDGRVEAIKEALMAHGLGNRVSVMSYSAKFASCFYGPFRDAAKSSPAFGDRRCYQLPPGARGLALRAVDRDVREGADMLMVKPGMPYLDIVREVKDKHPDLPLAVYHVSGEFAMLWHGAQAGAFDLKAAVLEAMTAFRRAGADIIITYYTPQLLQWLKEE

相同性解析による交差性が予測される生物種
Full Name aminolevulinate, delta-, dehydratase
Calculated molecular weight36kDa，330aa
Observed molecular weight 36-39 kDa
GenBank accession numberNM_000031.5
Gene Symbol ALAD
Gene ID (NCBI) 210
RRIDAB_3670079
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP13716
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Delta-aminolevulinic acid dehydratase (ALAD) is also named as Porphobilinogen synthase. ALAD, encoded by the moonlighting gene ALAD, not only participates in the second step of heme biosynthesis, but also inhibits the 26S proteasome (PMID: 35318110). ALAD is involved in tumor progression. For example, ALAD2 allele (ALAD polymorphism) is related to an increased risk for meningioma, and downregulation of ALAD is related to poor prognosis of patients with breast cancer (PMID: 16140629, PMID: 28403546).

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