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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AKTIP Polyclonal antibody (14860-1-AP)

    WB analysis of HepG2 using 14860-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14860-1-AP (AKTIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using AKTIP Polyclonal antibody (14860-1-AP)

    IP experiment of HepG2 using 14860-1-AP

    IP result of anti-AKTIP (IP:14860-1-AP, 4ug; Detection:14860-1-AP 1:300) with HepG2 cells lysate 1680 ug.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using AKTIP Polyclonal antibody (14860-1-AP)

    IF Staining of SH-SY5Y using 14860-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14860-1-AP (AKTIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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AKTIP Polyclonal antibody

AKTIP Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 14860-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

FTS, Ft1, AKT-interacting protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14860-1-AP targets AKTIP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKTIP fusion protein Ag6662 相同性解析による交差性が予測される生物種
Full Name AKT interacting protein
Calculated molecular weight 32 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001134
Gene Symbol AKTIP
Gene ID (NCBI) 64400
RRIDAB_2878087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8T0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AKTIP antibody 14860-1-APDownload protocol
IF protocol for AKTIP antibody 14860-1-APDownload protocol
IP protocol for AKTIP antibody 14860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

AKTIP loss is enriched in ERα-positive breast cancer for tumorigenesis and confers endocrine resistance

Authors - Angel S N Ng
  • KD Validated
View Article