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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using AKT Polyclonal antibody (10176-2-AP)

    WB analysis of MCF-7 using 10176-2-AP

    WB result of AKT antibody (10176-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected MCF-7 cells.

  • Western Blot (WB) analysis of HeLa cells using AKT Polyclonal antibody (10176-2-AP)

    WB analysis of HeLa cells using 10176-2-AP

    siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.

  • Western Blot (WB) analysis of various lysates using AKT Polyclonal antibody (10176-2-AP)

    WB analysis using 10176-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AKT Polyclonal antibody (10176-2-AP)

    WB analysis using 10176-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AKT Polyclonal antibody (10176-2-AP)

    WB analysis using 10176-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using AKT Polyclonal antibody (10176-2-AP)

    WB analysis of A549 using 10176-2-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:500.

  • Immunoprecipitation (IP) experiment of HeLa cells using AKT Polyclonal antibody (10176-2-AP)

    IP experiment of HeLa using 10176-2-AP

    IP result of anti-AKT (IP:10176-2-AP, 4ug; Detection:10176-2-AP 1:15000) with HeLa cells lysate 1085 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Polyclonal antibody (10176-2-AP)

    IHC staining of human breast cancer using 10176-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Polyclonal antibody (10176-2-AP)

    IHC staining of human breast cancer using 10176-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AKT Polyclonal antibody (10176-2-AP)

    IF Staining of MCF-7 using 10176-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AKT antibody (10176-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

AKT Polyclonal antibody

AKT Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 10176-2-AP
Publications(2308)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

AKT 1, AKT1, EC:2.7.11.1, PKB, PKB ALPHA

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, C2C12 cells, mouse brain tissue, rat brain tissue, Jurkat cells, RAW 264, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10176-2-AP targets AKT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, monkey, chicken, sheep, goat, zebra finches
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0213

Product name: Recombinant human AKT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-224 aa of BC000479

Sequence: MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLC

相同性解析による交差性が予測される生物種
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight 56-62 kDa
GenBank accession numberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
RRIDAB_2224574
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP31749
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Protocols

Product Specific Protocols
IF protocol for AKT antibody 10176-2-APDownload protocol
IHC protocol for AKT antibody 10176-2-APDownload protocol
IP protocol for AKT antibody 10176-2-APDownload protocol
WB protocol for AKT antibody 10176-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Overexpression of STX11 alleviates pulmonary fibrosis by inhibiting fibroblast activation via the PI3K/AKT/mTOR pathway

Authors - Guichuan Huang
View Article
humanWB

J Hematol Oncol

A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties.

Authors - Shuiqing Liu
View Article
humanWB

J Hematol Oncol

Long non-coding RNA-SNHG7 acts as a target of miR-34a to increase GALNT7 level and regulate PI3K/Akt/mTOR pathway in colorectal cancer progression.

Authors - Yang Li
View Article
humanWB,IP

J Hematol Oncol

A cytoplasmic long noncoding RNA LINC00470 as a new AKT activator to mediate glioblastoma cell autophagy.

Authors - Changhong Liu
View Article
humanWB,IHC

Mol Cancer

EIF4A3/circPTGR1/miR-4725-5p positive- feedback loop promotes colorectal cancer progression via FAK/AKT signaling pathway.

Authors - Yi Dong
View Article
humanIHC

Mol Cancer

Circular RNA circSLC8A1 acts as a sponge of miR-130b/miR-494 in suppressing bladder cancer progression via regulating PTEN.

Authors - Qun Lu
View Article
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