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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKR7A3 Polyclonal antibody

AKR7A3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13209-1-AP

Synonyms

AFAR2, AFB1 aldehyde reductase 2, AFB1 AR 2, AKR7A3

Validation Data Gallery

Applications:
  • WB analysis of human liver using 13209-1-AP

    WB analysis of human liver using 13209-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 13209-1-AP

    WB analysis of HepG2 using 13209-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 13209-1-AP

    WB analysis of NIH/3T3 using 13209-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 13209-1-AP

    WB analysis of human brain using 13209-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman liver tissue, HepG2 cells, human brain tissue, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13209-1-AP targets AKR7A3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR7A3 fusion protein Ag3991 相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase)
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight 37 kDa and 55-60 kDa
GenBank accession numberBC025709
Gene symbol AKR7A3
Gene ID (NCBI) 22977
RRIDAB_2224549
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AKR7A3 antibody 13209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Toxicol Sci

Generation of A New Model Rat: Nrf2 Knockout Rats Are Sensitive to Aflatoxin B1 Toxicity.

Authors - Keiko Taguchi
View Article