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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using AKR7A2 Polyclonal antibody (10730-1-AP)

    WB analysis of mouse liver using 10730-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AKR7A2 Polyclonal antibody (10730-1-AP)

    WB analysis of HEK-293 using 10730-1-AP

    WB result of AKR7A2 antibody (10730-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HepG2 cells using AKR7A2 Polyclonal antibody (10730-1-AP)

    WB analysis of HepG2 using 10730-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using AKR7A2 Polyclonal antibody (10730-1-AP)

    WB analysis of A431 using 10730-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AKR7A2 Polyclonal antibody (10730-1-AP)

    IHC staining of human liver cancer using 10730-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10730-1-AP (AKR7A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AKR7A2 Polyclonal antibody (10730-1-AP)

    IHC staining of human liver cancer using 10730-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10730-1-AP (AKR7A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

AKR7A2 Polyclonal antibody

AKR7A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10730-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, A431 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10730-1-AP targets AKR7A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR7A2 fusion protein Ag1049 相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
Calculated molecular weight 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC007352
Gene Symbol AKR7A2
Gene ID (NCBI) 8574
RRIDAB_2224439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43488
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells (PMID:22001351).

Protocols

Product Specific Protocols
WB protocol for AKR7A2 antibody 10730-1-APDownload protocol
IHC protocol for AKR7A2 antibody 10730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Environ Toxicol

Naringin alleviates acetaminophen-induced acute liver injury by activating Nrf2 via CHAC2 upregulation.

Authors - Xiaohan Zhai
View Article
humanWB,IHC

Pancreatology

Proteomic analysis of pancreatic ductal adenocarcinoma compared with normal adjacent pancreatic tissue and pancreatic benign cystadenoma.

Authors - Cui Yazhou Y
View Article