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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using AKR1CL2 Polyclonal antibody (20525-1-AP)

    WB analysis of mouse testis using 20525-1-AP (same clone as 20525-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20525-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW264.7 using AKR1CL2 Polyclonal antibody (20525-1-AP)

    WB analysis of RAW264.7 using 20525-1-AP (same clone as 20525-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20525-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using AKR1CL2 Polyclonal antibody (20525-1-AP)

    WB analysis of HT-1080 using 20525-1-AP (same clone as 20525-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20525-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AKR1CL2 Polyclonal antibody (20525-1-AP)

    IF Staining of HeLa using 20525-1-AP (same clone as 20525-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AKR1CL2 antibody (20525-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 20525-1-PBS in a different storage buffer formulation.

AKR1CL2 Polyclonal antibody, PBS Only

AKR1CL2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 20525-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Aldo-keto reductase family 1 member C-like protein 2, AKRDC1, AKR1E2, AKR1CL 2, AF reductase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20525-1-PBS targets AKR1CL2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14381

Product name: Recombinant human AKR1CL2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-307 aa of BC002862

Sequence: MGDIPAVGLSSWKASPGKVTEAVKEAIDAGYRHFDCAYFYHNEREVGAGIRCKIKEGAVRREDLFIATKLWCTCHKKSLVETACRKSLKALKLNYLDLYLIHWPMGFKPPHPEWIMSCSELSFCLSHPRVQDLPLDESNMVIPSDTDFLDTWEAMEDLVITGLVKNIGVSNFNHEQLERLLNKPGLRFKPLTNQIECHPYLTQKNLISFCQSRDVSVTAYRPLGGSCEGVDLIDNPVIKRIAKEHGKSPAQILIRFQIQRNVIVIPGSITPSHIKENIQVFDFELTQHDMDNILSLNRNLRLAMFPM

相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 1, member C-like 2
Calculated molecular weight 320 aa, 37 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002862
Gene Symbol AKR1CL2
Gene ID (NCBI) 83592
RRIDAB_10694285
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JD6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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