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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKR1C3 Polyclonal antibody (11194-1-AP)

    WB analysis using 11194-1-AP (same clone as 11194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11194-1-AP (AKR1C3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11194-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using AKR1C3 Polyclonal antibody (11194-1-AP)

    IP experiment of HepG2 using 11194-1-AP (same clone as 11194-1-PBS)

    IP result of anti-AKR1C3 (IP:11194-1-AP, 4ug; Detection:11194-1-AP 1:3000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 11194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using AKR1C3 Polyclonal antibody (11194-1-AP)

    IHC staining of human prostate cancer using 11194-1-AP (same clone as 11194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11194-1-AP (AKR1C3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using AKR1C3 Polyclonal antibody (11194-1-AP)

    IHC staining of human prostate cancer using 11194-1-AP (same clone as 11194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11194-1-AP (AKR1C3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11194-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using AKR1C3 Polyclonal antibody (11194-1-AP)

    IF Staining of A549 using 11194-1-AP (same clone as 11194-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using AKR1C3 antibody (11194-1-AP) at dilution of 1:225 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11194-1-PBS in a different storage buffer formulation.

AKR1C3 Polyclonal antibody, PBS Only

AKR1C3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11194-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11194-1-PBS targets AKR1C3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1674

Product name: Recombinant human AKR1C3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-323 aa of BC019230

Sequence: MDSKHQCVKLNDGHFMPVLGFGTYAPPEVPRSKALEVTKLAIEAGFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSTFHRPELVRPALENSLKKAQLDYVDLYLIHSPMSLKPGEELSPTDENGKVIFDIVDLCTTWEAMEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNRSKLLDFCKSKDIVLVAYSALGSQRDKRWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTAEDMKAIDGLDRNLHYFNSDSFASHPNYPYSDEY

相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
Calculated molecular weight 323 aa, 37 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC019230
Gene Symbol AKR1C3
Gene ID (NCBI) 8644
RRIDAB_2224414
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP42330
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism. The rat kidney possesses a dimeric form of 75 kDa(PMID:18574251).

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