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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKR1B10 Monoclonal antibody (68327-1-Ig)

    WB analysis using 68327-1-Ig (same clone as 68327-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat stomach tissue using AKR1B10 Monoclonal antibody (68327-1-Ig)

    WB analysis of rat stomach using 68327-1-Ig (same clone as 68327-1-PBS)

    rat stomach tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68327-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig small intestine tissue using AKR1B10 Monoclonal antibody (68327-1-Ig)

    WB analysis of pig small intestine using 68327-1-Ig (same clone as 68327-1-PBS)

    pig small intestine tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68327-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using AKR1B10 Monoclonal antibody (68327-1-Ig)

    IF Staining of A549 using 68327-1-Ig (same clone as 68327-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using AKR1B10 antibody (68327-1-Ig, Clone: 5A4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68327-1-PBS in a different storage buffer formulation.

AKR1B10 Monoclonal antibody, PBS Only

AKR1B10 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68327-1-PBS
Clone No.5A4G5

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HIS, HSI, SI reductase, Small intestine reductase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68327-1-PBS targets AKR1B10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Pig, Rat samples.

Tested Reactivity Human, Pig, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24406

Product name: Recombinant human AKR1B10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-316 aa of BC008837

Sequence: MATFVELSTKAKMPIVGLGTWKSPLGKVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWRACNVLQSSHLEDYPFDAEY

相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 1, member B10 (aldose reductase)
Calculated molecular weight 316 aa, 36 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC008837
Gene Symbol AKR1B10
Gene ID (NCBI) 57016
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO60218
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Aldo-keto reductase family 1 member B10 (AKR1B10), which belongs to the AKR superfamily, consists of 316 amino acids and its gene is located on chromosome 7q33. AKR1B10 was initially identified in liver cancer. It is highly expressed in the small intestine, colon, and adrenal gland.

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