Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
23785-1-AP targets AKNA in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AKNA fusion protein Ag20662 相同性解析による交差性が予測される生物種 |
| Full Name | AT-hook transcription factor |
| Calculated molecular weight | 155 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC055285 |
| Gene Symbol | AKNA |
| Gene ID (NCBI) | 80709 |
| RRID | AB_2879324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q7Z591 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AKNA also named as AT hook transcription factor is a 1439 amino-acids protein. AKNA, encodes a lymphoid-specific AT-hook transcription factor that is highly expressed by B and T lymphocytes. It coordinately regulates expression of the CD40 receptor and its ligand (CD154), two molecules that are critical for antigen-dependent B-cell development. AKNA expresses at least nine distinct transcripts, some of which are expressed in a tissue-specific manner. These transcripts are products of alternative splicing, alternative promoter usage, and alternative polyadenylation. The alternative AKNA transcripts are predicted to encode overlapping protein isoforms, some of which (p70 and p100) (PMID:15869410)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKNA antibody 23785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Res Int AKNA Is a Potential Prognostic Biomarker in Gastric Cancer and Function as a Tumor Suppressor by Modulating EMT-Related Pathways. |