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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKD2 Polyclonal antibody (27554-1-AP)

    WB analysis using 27554-1-AP (same clone as 27554-1-PBS)

    Various lysates lysate were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using AKD2 Polyclonal antibody (27554-1-AP)

    WB analysis of SMMC-7721 using 27554-1-AP (same clone as 27554-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using AKD2 Polyclonal antibody (27554-1-AP)

    WB analysis of HuH-7 using 27554-1-AP (same clone as 27554-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using AKD2 Polyclonal antibody (27554-1-AP)

    WB analysis of HepG2 using 27554-1-AP (same clone as 27554-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using AKD2 Polyclonal antibody (27554-1-AP)

    IHC staining of human heart using 27554-1-AP (same clone as 27554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using AKD2 Polyclonal antibody (27554-1-AP)

    IHC staining of human heart using 27554-1-AP (same clone as 27554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AKD2 Polyclonal antibody (27554-1-AP)

    IHC staining of human liver cancer using 27554-1-AP (same clone as 27554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AKD2 Polyclonal antibody (27554-1-AP)

    IHC staining of human liver cancer using 27554-1-AP (same clone as 27554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27554-1-PBS in a different storage buffer formulation.

AKD2 Polyclonal antibody, PBS Only

AKD2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27554-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

AK9, Adenylate kinase domain-containing protein 1, Adenylate kinase domain-containing protein 2, AK 9, AKD1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27554-1-PBS targets AKD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25805

Product name: Recombinant human AKD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-421 aa of BC022031

Sequence: MTSQEKTEEYPFADIFDEDETERNFLLSKPVCFVVFGKPGVGKTTLARYITQAWKCIRVEALPILEEQIAAETESGVMLQSMLISGQSIPDELVIKLMLEKLNSPEVCHFGYIITEIPSLSQDAMTTLQQIELIKNLNLKPDVIINIKCPDYDLCQRISGQRQHNNTGYIYSRDQWDPEVIENHRKKKKEAQKDGKGEEEEEEEEQEEEEAFIAEMQMVAEILHHLVQRPEDYLENVENIVKLYKETILQTLEEVMAEHNPQYLIELNGNKPAEELFMIVMDRLKYLNLKRAAILTKLQGAEEEINDTMENDELFRTLASYKLIAPRYRWQRSKWGRTCPVNLKDGNIYSGLPDYSVSFLGKIYCLSSEEALKPFLLNPRPYLLPPMPGPPCKVFILGPQYSGKTTLCNMLAENYKGKVTN

相同性解析による交差性が予測される生物種
Full Name adenylate kinase domain containing 2
Calculated molecular weight221 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC022031
Gene Symbol AKD2
Gene ID (NCBI) 221264
RRIDAB_2880904
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5TCS8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111).

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