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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKD1 Polyclonal antibody

AKD1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23042-1-AP

Synonyms

AKD1

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 23042-1-AP

    WB analysis of HEK-293 using 23042-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23042-1-AP (AKD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 23042-1-AP

    IHC staining of human kidney using 23042-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23042-1-AP (AKD1 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human testis using 23042-1-AP

    IHC staining of human testis using 23042-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23042-1-AP (AKD1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 23042-1-AP

    IHC staining of human testis using 23042-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23042-1-AP (AKD1 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human kidney using 23042-1-AP

    IHC staining of human kidney using 23042-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23042-1-AP (AKD1 Antibody) at dilution of 1:200 (under 10x lens).

  • IF Staining of HEK-293 using 23042-1-AP

    IF Staining of HEK-293 using 23042-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 23042-1-AP (AKD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23042-1-AP is AKD1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKD1 fusion protein Ag19038 相同性解析による交差性が予測される生物種
Full Name adenylate kinase domain containing 1
Calculated molecular weight 1911 aa, 221 kDa
Observed molecular weight 40-49 kDa
GenBank accession numberBC153055
Gene symbol AKD1
Gene ID (NCBI) 401271
RRIDAB_2879201
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AKD1 antibody 23042-1-APDownload protocol
IHC protocol for AKD1 antibody 23042-1-APDownload protocol
IF protocol for AKD1 antibody 23042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols