Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HL-60 cells, HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31542-1-AP targets AKAP8 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35816 Product name: Recombinant human AKAP8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 501-692 aa of NM_005858.3 Sequence: SVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE 相同性解析による交差性が予測される生物種 |
| Full Name | A kinase (PRKA) anchor protein 8 |
| Calculated molecular weight | 76kDa，692aa |
| Observed molecular weight | 95 kDa |
| GenBank accession number | NM_005858.3 |
| Gene Symbol | AKAP8 |
| Gene ID (NCBI) | 10270 |
| RRID | AB_3670031 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O43823 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AKAP8 (A-kinase anchor protein 8), also called AKAP95, is a A-kinase anchor protein that directs activity of protein kinase A by tethering the enzyme near its physiologic substrates (PMID: 9473338; 10601332). It is associated with chromatin and nuclear matrix which has a remarkable activity in promoting chromatin transcription (PMID: 31980632), and it is also required for cell cycle G2/M transition and histone deacetylation during mitosis (PMID: 16980585).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKAP8 antibody 31542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |