Validation Data Gallery

Filter: AllWBIHC

WB analysis of Jurkat using 14622-1-AP (same clone as 14622-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 14622-1-AP (AKAP14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 14622-1-AP (same clone as 14622-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 14622-1-AP (AKAP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 14622-1-AP (same clone as 14622-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14622-1-PBS in a different storage buffer formulation.

AKAP14 Polyclonal antibody, PBS Only

AKAP14 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14622-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14622-1-PBS targets AKAP14 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6255 Product name: Recombinant human AKAP14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-197 aa of BC066357 Sequence: MSETQNSTSQKAMDEDNKAASQTMPNTQDKNYEDELTQVALALVEDVINYAVKIVEEERNPLKNIKWMTHGEFTVEKGLKQIDEYFSKCVSKKCWAHGVEFVERKDLIHSFLYIYYVHWSISTADLPVARISAGTYFTMKVSKTKPPDAPIVVSYVGDHQALVHRPGMVRFRENWQKNLTDAKYSFMESFPFLFNRV 相同性解析による交差性が予測される生物種
Full Name	A kinase (PRKA) anchor protein 14
Calculated molecular weight	23 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC066357
Gene Symbol	AKAP14
Gene ID (NCBI)	158798
RRID	AB_2225760
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86UN6
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 14622-1-AP (same clone as 14622-1-PBS)

WB analysis of human testis using 14622-1-AP (same clone as 14622-1-PBS)

IHC staining of human kidney using 14622-1-AP (same clone as 14622-1-PBS)

IHC staining of human kidney using 14622-1-AP (same clone as 14622-1-PBS)

IHC staining of human placenta using 14622-1-AP (same clone as 14622-1-PBS)

IHC staining of human placenta using 14622-1-AP (same clone as 14622-1-PBS)

IHC staining of human testis using 14622-1-AP (same clone as 14622-1-PBS)

IHC staining of human testis using 14622-1-AP (same clone as 14622-1-PBS)

IHC staining of human brain using 14622-1-AP (same clone as 14622-1-PBS)

IHC staining of human brain using 14622-1-AP (same clone as 14622-1-PBS)

IHC staining of human lung using 14622-1-AP (same clone as 14622-1-PBS)