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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKAP13 Polyclonal antibody

AKAP13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27947-1-AP

Synonyms

A kinase anchor protein 13, AKAP 13, AKAP Lbc, AKAP13, ARHGEF13, BRX, c lbc, HA 3, Ht31, LBC, LBC oncogene, Lymphoid blast crisis oncogene, p47, PROTO LB, PROTO LBC

Validation Data Gallery

Applications:
  • WB analysis using 27947-1-AP

    WB analysis using 27947-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27947-1-AP (AKAP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27947-1-AP

    IHC staining of human breast cancer using 27947-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKAP13 antibody included
  • IHC staining of human breast cancer using 27947-1-AP

    IHC staining of human breast cancer using 27947-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKAP13 antibody included
  • IHC staining of human colon cancer using 27947-1-AP

    IHC staining of human colon cancer using 27947-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKAP13 antibody included
  • IHC staining of human colon cancer using 27947-1-AP

    IHC staining of human colon cancer using 27947-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKAP13 antibody included
  • IHC staining of human lung cancer using 27947-1-AP

    IHC staining of human lung cancer using 27947-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKAP13 antibody included
  • IHC staining of human lung cancer using 27947-1-AP

    IHC staining of human lung cancer using 27947-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKAP13 antibody included

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AKAP13 Ready-To-Use IHC Kit. AKAP13 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27947-1-AP targets AKAP13 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP13 fusion protein Ag27486 相同性解析による交差性が予測される生物種
Full Name A kinase (PRKA) anchor protein 13
Calculated molecular weight 308 kDa
GenBank accession numberNM_001270546
Gene symbol AKAP13
Gene ID (NCBI) 11214
RRIDAB_2881017
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AKAP13 antibody 27947-1-APDownload protocol
IHC protocol for AKAP13 antibody 27947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols