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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKAP1 Recombinant antibody (85119-2-RR)

    WB analysis using 85119-2-RR (same clone as 85119-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85119-2-RR (AKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AKAP1 Recombinant antibody (85119-2-RR)

    IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85119-2-RR (AKAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AKAP1 Recombinant antibody (85119-2-RR)

    IHC staining of human colon cancer using 85119-2-RR (same clone as 85119-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85119-2-RR (AKAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AKAP1 Recombinant antibody (85119-2-RR)

    IF Staining of HepG2 using 85119-2-RR (same clone as 85119-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AKAP1 Recombinant antibody (85119-2-RR)

    IF Staining of MCF-7 using 85119-2-RR (same clone as 85119-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using AKAP1 Recombinant antibody (85119-2-RR)

    FC experiment of HEK-293T using 85119-2-RR (same clone as 85119-2-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.13 ug AKAP1 Recombinant antibody (85119-2-RR, Clone:242763A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85119-2-RR

    Affinity and Kinetic Characterization of 85119-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85119-2-RR against Human AKAP1 were performed. The affinity constant is 3.62 nM.

AKAP1 Recombinant monoclonal antibody, PBS Only

AKAP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85119-2-PBS
Clone No.242763A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242763A5, AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85119-2-PBS targets AKAP1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AKAP1 fusion protein Ag8037 相同性解析による交差性が予測される生物種
Full Name A kinase (PRKA) anchor protein 1
Calculated molecular weight 97 kDa
Observed molecular weight149 kDa
GenBank accession numberBC000729
Gene Symbol AKAP1
Gene ID (NCBI) 8165
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ92667
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.