Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 15618-1-AP (same clone as 15618-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
WB analysis of human liver using 15618-1-AP (same clone as 15618-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IP experiment of PC-3 using 15618-1-AP (same clone as 15618-1-PBS)
IP result of anti-AKAP1 (IP:15618-1-AP, 4ug; Detection:15618-1-AP 1:2000) with PC-3 cells lysate 1800ug. This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 15618-1-AP (same clone as 15618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 15618-1-AP (same clone as 15618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 15618-1-AP (same clone as 15618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 15618-1-AP (same clone as 15618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 15618-1-AP (same clone as 15618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 15618-1-AP (same clone as 15618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IHC staining of human small intestine using 15618-1-AP (same clone as 15618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IHC staining of human small intestine using 15618-1-AP (same clone as 15618-1-PBS)
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 15618-1-AP (same clone as 15618-1-PBS)
Immunofluorescent analysis of MCF-7 cells, using AKAP1 antibody 15618-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15618-1-PBS in a different storage buffer formulation.

AKAP1 Polyclonal antibody, PBS Only

AKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15618-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

D-AKAP-1, DAKAP1, A-kinase anchor protein 149 kDa, A-kinase anchor protein 1, mitochondrial, AKAP 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15618-1-PBS targets AKAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8037 Product name: Recombinant human AKAP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 31-351 aa of BC000729 Sequence: GHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQI 相同性解析による交差性が予測される生物種
Full Name	A kinase (PRKA) anchor protein 1
Calculated molecular weight	97 kDa
Observed molecular weight	149 kDa
GenBank accession number	BC000729
Gene Symbol	AKAP1
Gene ID (NCBI)	8165
RRID	AB_2225484
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92667
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15618-1-AP (same clone as 15618-1-PBS)

WB analysis of human liver using 15618-1-AP (same clone as 15618-1-PBS)

IP experiment of PC-3 using 15618-1-AP (same clone as 15618-1-PBS)

IHC staining of human lung cancer using 15618-1-AP (same clone as 15618-1-PBS)

IHC staining of human lung cancer using 15618-1-AP (same clone as 15618-1-PBS)

IHC staining of human prostate cancer using 15618-1-AP (same clone as 15618-1-PBS)

IHC staining of human prostate cancer using 15618-1-AP (same clone as 15618-1-PBS)

IHC staining of human colon cancer using 15618-1-AP (same clone as 15618-1-PBS)

IHC staining of human colon cancer using 15618-1-AP (same clone as 15618-1-PBS)

IHC staining of human small intestine using 15618-1-AP (same clone as 15618-1-PBS)