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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis using 12562-1-AP (same clone as 12562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis using 12562-1-AP (same clone as 12562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using AK3 Polyclonal antibody (12562-1-AP)

    IP experiment of mouse kidney using 12562-1-AP (same clone as 12562-1-PBS)

    IP result of anti-AK3 (IP:12562-1-AP, 3ug; Detection:12562-1-AP 1:500) with mouse kidney tissue lysate 4400ug. This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using AK3 Polyclonal antibody (12562-1-AP)

    IHC staining of human pancreas cancer using 12562-1-AP (same clone as 12562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12562-1-AP (AK3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using AK3 Polyclonal antibody (12562-1-AP)

    IHC staining of human heart using 12562-1-AP (same clone as 12562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using AK3 Polyclonal antibody (12562-1-AP)

    IHC staining of human heart using 12562-1-AP (same clone as 12562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AK3 Polyclonal antibody (12562-1-AP)

    IF Staining of HepG2 using 12562-1-AP (same clone as 12562-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AK3 antibody (12562-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12562-1-PBS in a different storage buffer formulation.

AK3 Polyclonal antibody, PBS Only

AK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12562-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Adenylate kinase 3 alpha-like 1, AK 3, AK3L1, AK6, AKL3L

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12562-1-PBS targets AK3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3146

Product name: Recombinant human AK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-227 aa of BC013771

Sequence: MGASARLLRAVIMGAPGSGKGTVSSRITTHFELKHLSSGDLLRDNMLRGTEIGVLAKAFIDQGKLIPDDVMTRLALHELKNLTQYSWLLDGFPRTLPQAEALDRAYQIDTVINLNVPFEVIKQRLTARWIHPASGRVYNIEFNPPKTVGIDDLTGEPLIQREDDKPETVIKRLKAYEDQTKPVLEYYQKKGVLETFSGTETNKIWPYVYAFLQTKVPQRSQKASVTP

相同性解析による交差性が予測される生物種
Full Name adenylate kinase 3
Calculated molecular weight 227 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC013771
Gene Symbol AK3
Gene ID (NCBI) 50808
RRIDAB_2305373
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIJ7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.

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