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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AK1 Polyclonal antibody (14978-1-AP)

    WB analysis using 14978-1-AP (same clone as 14978-1-PBS)

    mouse heart lysates were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AK1 Polyclonal antibody (14978-1-AP)

    WB analysis using 14978-1-AP (same clone as 14978-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using AK1 Polyclonal antibody (14978-1-AP)

    WB analysis of mouse skeletal muscle using 14978-1-AP (same clone as 14978-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using AK1 Polyclonal antibody (14978-1-AP)

    WB analysis of human heart using 14978-1-AP (same clone as 14978-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using AK1 Polyclonal antibody (14978-1-AP)

    WB analysis of human heart using 14978-1-AP (same clone as 14978-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using AK1 Polyclonal antibody (14978-1-AP)

    IHC staining of mouse heart using 14978-1-AP (same clone as 14978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using AK1 Polyclonal antibody (14978-1-AP)

    IHC staining of mouse heart using 14978-1-AP (same clone as 14978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

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AK1 Polyclonal antibody, PBS Only

AK1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14978-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

adenylate kinase 1, Adenylate kinase isoenzyme 1, Adenylate monophosphate kinase, AK 1, ATP:AMP phosphotransferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14978-1-PBS targets AK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AK1 fusion protein Ag6890 相同性解析による交差性が予測される生物種
Full Name adenylate kinase 1
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC001116
Gene Symbol AK1
Gene ID (NCBI) 203
RRIDAB_2225148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00568
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AK1(Adenylate kinase isoenzyme 1), also known as ATP-AMP transphosphorylase 1. It is located in cytoplasm. This protein is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP 1. Exhibits nucleoside diphosphate kinase activity, catalyzing the production of ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donor 2. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP. The molecular weight of AK1 is 22 kDa.