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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of L02 using 22517-1-AP (same clone as 22517-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22517-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of mouse thymus using 22517-1-AP (same clone as 22517-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22517-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of human spleen using 22517-1-AP (same clone as 22517-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22517-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of human plasma using 22517-1-AP (same clone as 22517-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22517-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of U-937 using 22517-1-AP (same clone as 22517-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22517-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thymus tissue using AIRE Polyclonal antibody (22517-1-AP)

    IHC staining of human thymus using 22517-1-AP (same clone as 22517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 22517-1-AP (AIRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22517-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thymus tissue using AIRE Polyclonal antibody (22517-1-AP)

    IHC staining of human thymus using 22517-1-AP (same clone as 22517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 22517-1-AP (AIRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22517-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AIRE Polyclonal antibody (22517-1-AP)

    IF Staining of HeLa using 22517-1-AP (same clone as 22517-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 22517-1-AP (AIRE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22517-1-PBS in a different storage buffer formulation.

AIRE Polyclonal antibody, PBS Only

AIRE Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22517-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22517-1-PBS targets AIRE in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18249

Product name: Recombinant human AIRE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 96-348 aa of BC137270

Sequence: QKNEDECAVCRDGGELICCDGCPRAFHLACLSPPLREIPSGTWRCSSCLQATVQEVQPRAEEPRPQEPPVETPLPPGLRSAGEEVRGPPGEPLAGMDTTLVYKHLPAPPSAAPLPGLDSSALHPLLCVGPEGQQNLAPGARCGVCGDGTDVLRCTHCAAAFHWRCHFPAGTSRPGTGLRCRSCSGDVTPAPVEGVLAPSPARLAPGPAKDDTASHEPALHRDDLESLLSEHTFDGILQWAIQSMARPAAPFPS

相同性解析による交差性が予測される生物種
Full Name autoimmune regulator
Calculated molecular weight 545 aa, 58 kDa
Observed molecular weight 58-69 kDa
GenBank accession numberBC137270
Gene Symbol AIRE
Gene ID (NCBI) 326
RRIDAB_2879113
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDO43918
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The function of the protein is not well defined, however it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

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