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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIM2 Polyclonal antibody (20590-1-AP)

    WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AIM2 Polyclonal antibody (20590-1-AP)

    WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using AIM2 Polyclonal antibody (20590-1-AP)

    IHC staining of human testis using 20590-1-AP (same clone as 20590-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using AIM2 Polyclonal antibody (20590-1-AP)

    IHC staining of human testis using 20590-1-AP (same clone as 20590-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AIM2 Polyclonal antibody (20590-1-AP)

    IF Staining of MCF-7 using 20590-1-AP (same clone as 20590-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AIM2 antibody (20590-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20590-1-PBS targets AIM2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIM2 fusion protein Ag14520 相同性解析による交差性が予測される生物種
Full Name absent in melanoma 2
Calculated molecular weight 343 aa, 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC010940
Gene Symbol AIM2
Gene ID (NCBI) 9447
RRIDAB_10694420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14862
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria (PMID:22695634). Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity (PMID:16432157,20566831). It has been reported that AIM2 protein levels are significantly elevated in alveolar macrophages of patients with IPF and bleomycin-treated mice (PMID: 38078661). AIM2 inflammasome was also significantly activated, which was also confirmed in Aβ-treated microglial cells (PMID: 34861878)