Validation Data Gallery

Filter: AllWBIHC

WB analysis using 14468-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 14468-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse heart using 14468-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse testis using 14468-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14468-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of rat ovary using 14468-1-AP
rat ovary tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 14468-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 14468-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14468-1-AP (AIG1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary tumor using 14468-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14468-1-AP (AIG1 antibody) at dilution of 1:100 (under 10x lens).

AIG1 Polyclonal antibody

AIG1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14468-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AIG 1, AIG1, androgen induced 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, mouse heart tissue, mouse testis tissue, PC-3 cells, rat heart tissue, rat ovary tissue, mouse ovary tissue
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

14468-1-AP targets AIG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5836 Product name: Recombinant human AIG1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-238 aa of BC025278 Sequence: MALVPCQVLRMAILLSYCSILCNYKAIEMPSHQTYGGSWKFLTFIDLVIQAVFFGICVLTDLSSLLTRGSGNQEQERQLKKLISLRDWMLAVLAFPVGVFVVAVFWIIYAYDREMIYPKLLDNFIPGWLNHGMHTTVLPFILIEMRTSHHQYPSRSSGLTAICTFSVGYILWVCWVHHVTGMWVYPFLEHIGPGARIIFFGSTTILMNFLYLLGEVLNNYIWDTQKSMEEEKEKPKLE 相同性解析による交差性が予測される生物種
Full Name	androgen-induced 1
Calculated molecular weight	28 kDa
Observed molecular weight	28-32 kDa
GenBank accession number	BC025278
Gene Symbol	AIG1
Gene ID (NCBI)	51390
RRID	AB_2273794
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NVV5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for AIG1 antibody 14468-1-AP	Download protocol
IHC protocol for AIG1 antibody 14468-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nat Commun Inflammation causes insulin resistance in mice via interferon regulatory factor 3 (IRF3)-mediated reduction in FAHFA levels Authors - Shuai Yan View Article

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研究分野

Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14468-1-AP

WB analysis of PC-3 using 14468-1-AP

WB analysis of mouse heart using 14468-1-AP

WB analysis of mouse testis using 14468-1-AP

WB analysis of K-562 using 14468-1-AP

WB analysis of rat ovary using 14468-1-AP

WB analysis of rat heart using 14468-1-AP

IHC staining of human ovary tumor using 14468-1-AP