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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIFM3 Polyclonal antibody (14778-1-AP)

    WB analysis using 14778-1-AP (same clone as 14778-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14778-1-AP (AIFM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AIFM3 Polyclonal antibody (14778-1-AP)

    IHC staining of human ovary tumor using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AIFM3 Polyclonal antibody (14778-1-AP)

    IHC staining of human ovary tumor using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AIFM3 Polyclonal antibody (14778-1-AP)

    IHC staining of human colon cancer using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AIFM3 Polyclonal antibody (14778-1-AP)

    IHC staining of human colon cancer using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

AIFM3 Polyclonal antibody, PBS Only

AIFM3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14778-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:1.-.-.-, Apoptosis-inducing factor-like protein, Apoptosis-inducing factor 3, Apoptosis inducing factor 3, AIFL

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14778-1-PBS targets AIFM3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6278

Product name: Recombinant human AIFM3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 355-598 aa of BC032485

Sequence: AHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFYMQTEVSELRGQEGKLKEVVLKSSKVVRADVCVVGIGAVPATGFLRQSGIGLDSRGFIPVNKMMQTNVPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHAQGRVAAQNMLAQEAEMSTVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKGDEVIAVASMNYDPIVSKVAEVLASGRAIRKREVETGDMSWLTGKGS

相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 3
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC032485
Gene Symbol AIFM3
Gene ID (NCBI) 150209
RRIDAB_2258090
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NN9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) is a mitochondrial protein/flavoenzyme and its gene is located on chromosome 22q11.21. Mature human AIFM3 protein consists of 598 amino acids with a molecular weight of 66 kDa and is predominantly localized in the mitochondria. AIFM3 is widely expressed in many tissues, and breast cancer tissues and cholangiocarcinoma tissue (PMID: 32664187).

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