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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIFM2/ FSP1 Polyclonal antibody (20886-1-AP)

    WB analysis using 20886-1-AP (same clone as 20886-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AIFM2/ FSP1 Polyclonal antibody (20886-1-AP)

    WB analysis using 20886-1-AP (same clone as 20886-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AIFM2/ FSP1 Polyclonal antibody (20886-1-AP)

    WB analysis using 20886-1-AP (same clone as 20886-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of L02 cells using AIFM2/ FSP1 Polyclonal antibody (20886-1-AP)

    IP experiment of L02 using 20886-1-AP (same clone as 20886-1-PBS)

    IP result of anti-AIFM2/ FSP1 (IP:20886-1-AP, 4ug; Detection:20886-1-AP 1:3000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AIFM2/ FSP1 Polyclonal antibody (20886-1-AP)

    IHC staining of mouse liver using 20886-1-AP (same clone as 20886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AIFM2/ FSP1 Polyclonal antibody (20886-1-AP)

    IHC staining of mouse liver using 20886-1-AP (same clone as 20886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20886-1-AP (AIFM2/ FSP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using AIFM2/ FSP1 Polyclonal antibody (20886-1-AP)

    IF Staining of A549 using 20886-1-AP (same clone as 20886-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AIFM2/ FSP1 antibody (20886-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20886-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AIFM2/ FSP1 Polyclonal antibody, PBS Only

AIFM2/ FSP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20886-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

AIFM2, AMID, FSP1, AIFM 2, Apoptosis inducing factor 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20886-1-PBS targets AIFM2/ FSP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13412

Product name: Recombinant human AIFM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-373 aa of BC023601

Sequence: MGSQVSVESGALHVVIVGGGFGGIAAASQLQALNVPFMLVDMKDSFHHNVAALRASVETGFAKKTFISYSVTFKDNFRQGLVVGIDLKNQMVLLQGGEALPFSHLILATGSTGPFPGKFNEVSSQQAAIQAYEDMVRQVQRSRFIVVVGGGSAGVEMAAEIKTEYPEKEVTLIHSQVALADKELLPSVRQEVKEILLRKGVQLLLSERVSNLEELPLNEYREYIKVQTDKGTEVATNLVILCTGIKINSSAYRKAFESRLASSGALRVNEHLQVEGHSNVYAIGDCADVRTPKMAYLAGLHANIAVANIVNSVKQRPLQAYKPGALTFLLSMGRNDGVGQISGFYVGRLMVRLTKSRDLFVSTSWKTMRQSPP

相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Calculated molecular weight 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC023601
Gene Symbol AIFM2/ FSP1
Gene ID (NCBI) 84883
RRIDAB_2878756
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRQ8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis-associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis-Inducing Factor), a mitochondrion-associated enzyme that relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

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