• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using AIF Monoclonal antibody (67791-1-Ig)

    WB analysis using 67791-1-Ig (same clone as 67791-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67791-1-Ig (AIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AIF Monoclonal antibody (67791-1-Ig)

    WB analysis of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    WB result of AIF antibody (67791-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIF transfected HeLa cells. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using AIF Monoclonal antibody (67791-1-Ig)

    IHC staining of human stomach cancer using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of HCT 116 using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AIF Monoclonal antibody, PBS Only

AIF Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67791-1-PBS
Clone No.8C12B2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, canine

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

AIFM1, PDCD8, EC:1.6.99.-, Apoptosis-inducing factor 1, mitochondrial

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67791-1-PBS targets AIF in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.

Tested Reactivity human, mouse, rat, pig, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12400

Product name: Recombinant human AIF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 259-609 aa of BC111065

Sequence: TPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED

相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 1
Calculated molecular weight 609 aa, 66 kDa
Observed molecular weight 67 kDa, 57 kDa
GenBank accession numberBC111065
Gene Symbol AIF
Gene ID (NCBI) 9131
RRIDAB_2918555
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95831
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).

||
New chat Able
正在加载，请稍候...