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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AHCY Monoclonal antibody, PBS Only

AHCY Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9B10

Cat no : 66019-1-PBS

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66019-1-Ig (same clone as 66019-1-PBS)

    WB analysis of MCF-7 using 66019-1-Ig (same clone as 66019-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • WB analysis of L02 using 66019-1-Ig (same clone as 66019-1-PBS)

    WB analysis of L02 using 66019-1-Ig (same clone as 66019-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    WB analysis of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHCY antibody included
  • IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHCY antibody included
  • IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

    IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHCY antibody included
  • IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

    IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHCY antibody included
  • IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AHCY antibody (66019-1-Ig, Clone: 1F9B10 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy AHCY Ready-To-Use IHC Kit. AHCY primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66019-1-PBS targets AHCY in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AHCY fusion protein Ag18036 相同性解析による交差性が予測される生物種
Full Name S-adenosylhomocysteine hydrolase
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC011606
Gene symbol AHCY
Gene ID (NCBI) 191
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).