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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGTR2 Polyclonal antibody (30963-1-AP)

    WB analysis using 30963-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30963-1-AP (AGTR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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AGTR2 Polyclonal antibody

AGTR2 Polyclonal Antibody for WB, ELISA
Cat No. 30963-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Type 2 angiotensin II receptor, ATGR2, AT2, Angiotensin II type 2 receptor, AGTR 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30963-1-AP targets AGTR2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGTR2 fusion protein Ag33887 相同性解析による交差性が予測される生物種
Full Name angiotensin II receptor, type 2
Calculated molecular weight41 kDa
Observed molecular weight38 kDa
GenBank accession numberNM_000686
Gene Symbol AGTR2
Gene ID (NCBI) 186
RRIDAB_3669797
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP50052
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AGTR2 (angiotensin II type 2 receptor) is part of the renin-angiotensin signaling (RAS) pathway that has been widely studied for its role in blood pressure regulation and renal and cardiovascular health (PMID: 29937318). AGTR2 mediates the effects of angiotensin II on cellular differentiation and growth (PMID: 30455538).

Protocols

Product Specific Protocols
WB protocol for AGTR2 antibody 30963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols