Validation Data Gallery
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Tested Applications
| Positive WB detected in | SKOV-3 cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
Product Information
11967-1-AP targets AGR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2577 Product name: Recombinant human AGR3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC058284 Sequence: MMLHSALGLCLLLVTVSSNLAIAIKKEKRPPQTLSRGWGDDITWVQTYEEGLFYAQKSKKPLMVIHHLEDCQYSQALKKVFAQNEEIQEMAQNKFIMLNLMHETTDKNLSPDGQYVPRIMFVDPSLTVRADIAGRYSNRLYTYEPRDLPLLIENMKKALRLIQSEL 相同性解析による交差性が予測される生物種 |
| Full Name | anterior gradient homolog 3 (Xenopus laevis) |
| Calculated molecular weight | 166 aa, 19 kDa |
| Observed molecular weight | 19-25 kDa |
| GenBank accession number | BC058284 |
| Gene Symbol | AGR3 |
| Gene ID (NCBI) | 155465 |
| RRID | AB_2877809 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TD06 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AGR3 antibody 11967-1-AP | Download protocol |
| IHC protocol for AGR3 antibody 11967-1-AP | Download protocol |
| WB protocol for AGR3 antibody 11967-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer. | ||
Front Pharmacol AGR3 Regulates Airway Epithelial Junctions in Patients with Frequent Exacerbations of COPD. | ||
iScience Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium | ||
J Transl Med Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers | ||
Cell High-resolution spatially resolved proteomics of complex tissues based on microfluidics and transfer learning |
