Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human testis tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
16762-1-AP targets AGPAT6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10294 Product name: Recombinant human AGPAT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 206-456 aa of BC051377 Sequence: KEFMSKHVHLMCYRICVRALTAIITYHDRENRPRNGGICVANHTSPIDVIILASDGYYAMVGQVHGGLMGVIQRAMVKACPHVWFERSEVKDRHLVAKRLTEHVQDKSKLPILIFPEGTCINNTSVMMFKKGSFEIGATVYPVAIKYDPQFGDAFWNSSKYGMVTYLLRMMTSWAIVCSVWYLPPMTREADEDAVQFANRVKSAIARQGGLVDLLWDGGLKREKVKDTFKEEQQKLYSKMIVGNHKDRSRS 相同性解析による交差性が予測される生物種 |
| Full Name | 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta) |
| Calculated molecular weight | 456 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC051377 |
| Gene Symbol | AGPAT6 |
| Gene ID (NCBI) | 137964 |
| RRID | AB_1850878 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UL3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family. AGPAT6 is expressed in brown adipose tissue and mammary epithelium as well as many other tissues. A deficiency of AGPAT6 in mice results in resistance to dietinduced and genetic forms of obesity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AGPAT6 antibody 16762-1-AP | Download protocol |
| IHC protocol for AGPAT6 antibody 16762-1-AP | Download protocol |
| IP protocol for AGPAT6 antibody 16762-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves. | ||
EMBO Rep MTCH2 cooperates with MFN2 and lysophosphatidic acid synthesis to sustain mitochondrial fusion |