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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGPAT4 Polyclonal antibody (16562-1-AP)

    WB analysis using 16562-1-AP (same clone as 16562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16562-1-AP (AGPAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16562-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AGPAT4 Polyclonal antibody (16562-1-AP)

    WB analysis using 16562-1-AP (same clone as 16562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16562-1-AP (AGPAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AGPAT4 Polyclonal antibody (16562-1-AP)

    IHC staining of human lung cancer using 16562-1-AP (same clone as 16562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16562-1-AP (AGPAT4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AGPAT4 Polyclonal antibody (16562-1-AP)

    IHC staining of human lung cancer using 16562-1-AP (same clone as 16562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16562-1-AP (AGPAT4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16562-1-PBS in a different storage buffer formulation.

AGPAT4 Polyclonal antibody, PBS Only

AGPAT4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16562-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

1 AGP acyltransferase 4, 1 AGPAT 4, 1 AGPAT4, 1-acylglycerol-3-phosphate O-acyltransferase 4, 1-acyl-sn-glycerol-3-phosphate acyltransferase delta

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16562-1-PBS targets AGPAT4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9834

Product name: Recombinant human AGPAT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-300 aa of BC020209

Sequence: SGLIINTIQLFTLLLWPINKQLFRKINCRLSYCISSQLVMLLEWWSGTECTIFTDPRAYLKYGKENAIVVLNHKFEIDFLCGWSLSERFGLLGGSKVLAKKELAYVPIIGWMWYFTEMVFCSRKWEQDRKTVATSLQHLRDYPEKYFFLIHCEGTRFTEKKHEISMQVARAKGLPRLKHHLLPRTKGFAITVRSLRNVVSAVYDCTLNFRNNENPTLLGVLNGKKYHADLYVRRIPLEDIPEDDDECSAWLHKLYQEKDAFQEEYYRTGTFPETPMV

相同性解析による交差性が予測される生物種
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta)
Calculated molecular weight 378 aa, 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC020209
Gene Symbol AGPAT4
Gene ID (NCBI) 56895
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRZ5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AGPAT4 (Acylglycerophosphate acyltransferase 4), also known as LPAATδ (Lysophosphatidic acid acyltransferase delta), is a protein expressed mainly in brain and in muscle. AGPAT4 is characterized as a mitochondrial lysophosphatidic acid acyltransferase that regulates brain levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI) (PMID: 28807933). AGPAT4 is relatively abundant in murine renal white adipose tissue (WAT) and has been linked to the regulation of TAG production, the differentiation of adipocytes, and the modulation of various pathologies including lipodystrophy, diabetes, and hepatic steatosis (PMID: 28814640).

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