Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, K-562 cells, L02 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33759-1-AP targets AGK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37404 Product name: Recombinant human AGK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 93-423 aa of BC022777 Sequence: MDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGETSSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQTHQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEPDTISKGDFITIGSRKVRNPKLHVEGTECLQASQCTLLIPEGAGGSFSIDSEEYEAMPVEVKLLPRKLQFFCDPRKREQMLTSPTQ 相同性解析による交差性が予測される生物種 |
| Full Name | acylglycerol kinase |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC022777 |
| Gene Symbol | AGK |
| Gene ID (NCBI) | 55750 |
| RRID | AB_3743070 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q53H12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AGK (Acylglycerol Kinase), also designated as MULK (multi-substrate lipid kinase), is a member of the AGK protein family and functions as a lipid kinase localized to the inner mitochondrial membrane. This protein exhibits a distinctive dual functionality. First, acting as a lipid kinase, it catalyzes the phosphorylation of monoacylglycerol and diacylglycerol to yield lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively, thereby participating in phospholipid metabolism and lipid-mediated signal transduction. Second, independent of its kinase activity, AGK serves as a core subunit of the TIM22 complex, where it is responsible for the translocation and insertion of multiple mitochondrial carrier proteins (e.g., ANT1) into the inner mitochondrial membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AGK antibody 33759-1-AP | Download protocol |
| WB protocol for AGK antibody 33759-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |