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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGER/RAGE Recombinant antibody (83759-5-RR)

    WB analysis using 83759-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83759-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of mouse lung using 83759-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of mouse lung using 83759-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of rat lung using 83759-5-RR

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83759-5-RR (AGER/RAGE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of rat lung using 83759-5-RR

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83759-5-RR (AGER/RAGE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IF Staining of mouse lung using 83759-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (83759-5-RR, Clone: 240706C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IF Staining of mouse lung using 83759-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (83759-5-RR, Clone: 240706C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83759-5-RR

    Affinity and Kinetic Characterization of 83759-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83759-5-RR against Rat AGER/RAGE were performed. The affinity constant is 3.46 nM.

AGER/RAGE Recombinant monoclonal antibody

AGER/RAGE Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83759-5-RR
Clone No.240706C3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

AGER, RAGE, 240706C3, Advanced glycosylation end product-specific receptor

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, mouse heart tissue
Positive IHC detected inmouse lung tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83759-5-RR targets AGER/RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1135

Product name: Recombinant Rat AGER/RAGE protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 23-341 aa of

Sequence: GQNITARIGEPLMLSCKGAPKKPTQKLEWKLNTGRTEAWKVLSPQGDPWDSVARILPNGSLLLPAIGIVDEGTFRCRATNRLGKEVKSNYRVRVYQIPGKPEIVNPASELTANVPNKVGTCVSEGSYPAGTLSWHLDGKPLIPDGKGTVVKEETRRHPETGLFTLRSELTVTPAQGGTTPTYSCSFSLGLPRRRPLNTAPIQPRVREPLPPEGIQLLVEPEGGTVAPGGTVTLTCAISAQPPPQIHWIKDGTPLPLAPSPVLLLPEVGHEDEGIYSCVATHPSHGPQESPPVNIRVTETGDEGQAAGSVDGSGLGTLAL

相同性解析による交差性が予測される生物種
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight43 kDa
Observed molecular weight43 kDa
Gene Symbol Ager
Gene ID (NCBI) 81722
RRIDAB_3671355
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ63495
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).

Protocols

Product Specific Protocols
IF protocol for AGER/RAGE antibody 83759-5-RRDownload protocol
IHC protocol for AGER/RAGE antibody 83759-5-RRDownload protocol
WB protocol for AGER/RAGE antibody 83759-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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