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Validation Data Gallery

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  • Western Blot (WB) analysis of pig lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    WB analysis of pig lung using 66833-1-Ig (same clone as 66833-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 66833-1-Ig (AGER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    WB analysis of rat lung using 66833-1-Ig (same clone as 66833-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 66833-1-Ig (AGER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    IF Staining of mouse lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    IF Staining of mouse lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    IF Staining of rat lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AGER antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

AGER/RAGE Monoclonal antibody, PBS Only

AGER/RAGE Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66833-1-PBS
Clone No.3D4G11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

AGER, RAGE, 3D4G11, Advanced glycosylation end product-specific receptor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66833-1-PBS targets AGER/RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9657

Product name: Recombinant human AGER protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 26-340 aa of BC020669

Sequence: ITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGT

相同性解析による交差性が予測される生物種
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight 404 aa, 43 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC020669
Gene Symbol AGER
Gene ID (NCBI) 177
RRIDAB_2882176
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ15109
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

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